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ASTM International , 03/01/2013
Publisher: ASTM
File Format: PDF
$46.00$92.00
1.1This guide describes procedures for obtaining laboratory data concerning the adverse effects of a test material added to dilution water-but not to food-on certain species of freshwater and saltwater fishes during 28 to 120-day (depending on species) continuous exposure, beginning before hatch and ending after hatch, using the flow-through technique. This guide will probably be useful for conducting early life-stage toxicity tests with some other species of fish, although modifications might be necessary.
1.2Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting early life-stage toxicity tests with fishes.
1.3These procedures are applicable to all chemicals, either individually or in formulations, commercial products, or known mixtures, that can be measured accurately at the necessary concentrations in water. With appropriate modifications these procedures can be used to conduct tests on temperature, dissolved oxygen, and pH and on such materials as aqueous effluents (see Guide E1192), leachates, oils, particulate matter, sediments, and surface waters.
1.4This guide is arranged as follows:
Section
Referenced Documents
2
Terminology
3
Summary of Standard
4
Significance and Use
5.1
Hazards
6
Apparatus
7
Facilities
7.1
Construction Materials
7.2
Metering System
7.3
Test Chambers and Incubation Cups
7.4
Cleaning
7.5
Acceptability
7.6
Dilution Water
8
Requirements
8.1
Source
8.2
Treatment
8.3
Characterization
8.4
Test Material
9
General
9.1
Stock Solution
9.2
Test Concentration(s)
9.3
Test Organisms
10
Species
10.1
Age
10.2
10.3
Brood Stock
10.4
Handling
10.5
Procedure
11
Experimental Design
11.1
Dissolved Oxygen
11.2
Temperature
11.3
Beginning the Test
11.4
Thinning
11.5
Feeding
11.6
Duration of Test
11.7
Biological Data
11.8
Other Measurements
11.9
Analytical Methodology
12
Acceptability of Test
13
Calculation of Results
14
Documentation
15
Appendixes
Appendix X1Salmon, Trout, and Char
Appendix X2Northern pike
Appendix X3Fathead minnow
Appendix X4White sucker
Appendix X5Channel catfish
Appendix X6Bluegill
Appendix X7Gulf toadfish
Appendix X8Sheepshead minnow
Appendix X9Silversides
Appendix X10Statistical Guidance
Appendix X11.Striped Bass
1.5This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 6 and 9.
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