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ASTM International , 02/01/2016
Publisher: ASTM
File Format: PDF
$49.00$98.00
1.1This practice covers procedures for obtaining data concerning toxicity and other effects of a test material to a multi-trophic level freshwater community.
1.2These procedures also might be useful for studying the fate of test materials and transformation products, although modifications and additional analytical procedures might be necessary.
1.3Modification of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting multi-trophic level tests.
1.4This practice is arranged as follows:
Section
Referenced Documents
2
Terminology
3
Summary of Practice
4
Significance and Use
5
Apparatus
6
Facilities
6.1
Container
6.2
Equipment
6.3
Hazards
7
Microcosm Components
8
Medium
8.1
Medium Preparation
8.2
Sediment
8.3
Microcosm Assembly
8.4
Test Material
9
General
9.1
Stock Solution
9.2
Nutrient Control
9.3
Test Concentration(s)
9.4
Test Organisms
10
Algae
10.1
Animals
10.2
Specificity of Organisms
10.3
Sources
10.4
Algal Culture Maintenance
10.5
Animal Culture Maintenance
10.6
Procedure
11
Experimental Design
11.1
Inoculation
11.2
Culling
11.3
Addition of Test Material
11.4
Measurements
11.5
Reinoculations
11.6
Analytical Methodology
12
Data Processing
13
Calculations of Variables from Measurements
14
Statistical Analyses
15
Acceptability of Test
16
Interpretation of Results
17
Report
18
Annex
Annex A1
Appendices
Relationship of Media
Appendix X1
Statistical Guidance
Appendix X2
1.5The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.6This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 7.
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