AWWA ACE63225

This slide presentation outlines a study on mammalian cell toxicity of emerging drinking water disinfection byproducts (DBP). Objectives of the study include: U.S. Environmental Protection Agency (USEPA) provides ~50 mg samples of synthesized, analytical grade DBP; analyze the direct-acting cytotoxicity and genomic genotoxicity of the individual DBPs with Chinese hamster ovary (CHO) cells; determine the cytotoxic and genotoxic rank order of the DBPs; and, develop a quantitative and comparative DBP toxicity database. Topics outlined include: Impediments to the Development of a Quantitative and Comparative DBP Toxicity Database; after 32 years, DBPs are still considered emerging contaminants; Solutions to the Impediments for a Comparative Toxicology of DBPs; USEPA study titled, "The Occurrence of Disinfection Byproducts (DBPs) of Health Concern in Drinking Water: Results of a Nationwide DBP Study Occurrence Study"; USEPA Stage 2 DBP Rule; alternatives to chlorine disinfection; Emerging DBP Chemical Classes That Are Currently Being Analyzed; Chinese Hamster Ovary Cells; Mammalian Cell Chronic Cytotoxicity Assay; Cytotoxicity Absorbancy Data: Dibromoacetamide; CHO Cell Cytotoxicity of Haloacetic Acids, Emerging Haloacid DBPs, Halonitromethanes; Summary of DBPs Analyzed with the CHO Chronic Cytotoxicity Assay; Genomic DNA Damage Induced by Drinking Water Disinfection Byproducts; SCGE Analysis of Haloacetic Acids, emerging Haloacids, Halonitromethanes in CHO Cells; and, Comparative DBP Genotoxicity Database. Includes figures.