AWWA ACE70554

This powerpoint presentation begins by providing the research objective: to better understand the fate of manganese - whether from source water or treatment plant impurities - under conditions of biological filtration, and to improve the water industry's understanding of, and ability to control, manganese release or pass-through during biological treatment. Biofilm T-RFLP analysis results: T-RFLP analysis has been completed on 32 of the 41 samples received, with nine samples failing to amplify by PCR, apparently due to low DNA concentrations and/or PCR inhibitors in the DNA; T-RFLP is continuing on more reference control strains; statistical analysis of the T-RFLP profiles; the PCR tests were completed for Pedomicrobium and Leptothrix; quantitation of PCR results in progress; comparison of T-RFLP and PCR shows that T-RFLP peak 148 is not a reliable predictor of the presence of Pedomicrobium; comparison of T-RFLP and PCR shows that T-RFLP peaks 119, 138, and 470 are not reliable predictors of the presence of Leptothrix; and, T-RFLP peaks are often characteristic of several bacteria. The identity of members of the community contributing to a peak can only be resolved by specific PCR or 16S rRNA gene clone library analysis. Includes tables, figures.