• AWWA WQTC58785

AWWA WQTC58785

Using Ribosome Genesis as a Sensitive Indicator of Microbial Viability to Test Disinfection Efficacy

American Water Works Association , 11/02/2003

Publisher: AWWA

File Format: PDF

$12.00$24.00


The objective of this study was to demonstrate that ribosomal RNA-targeted techniques could be used to quantify viable microorganisms when determining disinfection efficacy. The authors have developed a new technique to simultaneously measure ribosomal RNA as well as precursor ribosomal RNA, a key intermediate in the production of ribosomes. Ribosomal RNA and precursor ribosomal RNA were measured simultaneously in cultures of Acinetobacter calcoaceticusT and Mycobacterium mucogenicum. The results demonstrate that precursor ribosomal RNA is a sensitive measure of viability. The authors believe this approach holds tremendous promise for verifying disinfection efficacy for microorganisms using molecular biology-based monitoring. In the future, coupling the rapid developments in molecular biology-based technology with the critical measurement of cellular viability via detection of ribosome genesis may change the way disinfectants are evaluated. Includes 12 references, figures.

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