AWWA WQTC65811

This powerpoint presentation begins by providing a brief history of Naegleria fowleri, a disease recognized at Adelaide (Australia) Children's Hospital in 1961; first cases in Florida appeared in 1966; N. fowleri named in 1970; the Centers for Disease Control (CDC) documented 24 U.S. fatal cases of PAM from 1989 - 2000; and, in 2002, two cases were reported in each state of Texas, Arizona, and Florida. Inhalation vs. ingestion of N. fowleri is presented, along with the three possible stages, infection risk, infectious dose, risk analysis, identification, Polymerase Chain Reaction "Primer", AWQC Methodology for Naegleria spp I - Processing of Environmental Samples, AWQC Methodology for Naegleria spp II - Speciation by PCR & Melting Analysis, Melting analysis of Naegleria spp from Peoria samples, Culture/PCR vs Direct PCR, Difference in Naegleria Primers, and Suggested Operational Practices for Drinking Water. Includes figures.