BS PD ISO/TS 21569-4:2016

BS PD ISO/TS 21569-4:2016 specifies a procedure for the detection of a DNA sequence of the promoter region of thenopaline synthase gene (P-nos) from Agrobacterium tumefaciens and a procedure for the detection of theDNA transition sequence between P-nos and the neomycin-phosphotransferase gene (nptII) from theTn5 transposon of Escherichia coli K12. The nos-promoter and the P-nos-nptII-construct are frequentlyfound in genetically modified plants. The P-nos and P-nos-nptII specific methods are based on realtimePCR and can be used for qualitative screening purposes. For identification and quantification of aspecific genetically modified plant (event) a follow-up analysis has to be carried out.The methods described are applicable for the analysis of DNA extracted from foodstuffs. They may alsobe suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. Theapplication of these methods requires the extraction of an adequate amount of amplifiable DNA fromthe relevant matrix.The DNA sequence amplified by the P-nos element-specific method can be detected in samples whichcontain DNA of the naturally occurring Ti-plasmid of A. tumefaciens. For this reason, it is necessary toconfirm a positive screening result. Further analyses are required using construct-specific or eventspecific methods.Cross References:ISO 21569ISO 21570ISO 21571:2005ISO 24276ISO 5725 ISO/IEC 17025ISO 16577